On the mitochondria, ribonucleic acid, acid phosphatase and polysaccharide of liver cells of a fish (Oryzias latipes) during starvation. III. On polysaccharide.

The whole observation was performed using an osseous fish (Oryzias latipes). The experiment was carried out from March to April in 1951. Fish were kept in aquaria, the water temperature of which was approximately 20°C. throughout the period. During the first two weeks all fish were fully fed, and then these fish were divided into two groups ; i.e. the starvation experiment group and the control. Individuals of the latter group were well fed as before. Those of the experimental group were kept in distilled water, which was changed every three days. On the tenth, the twentieth and the thirtieth day, livers were excised from living fish. And fixed in 70% alcohol. Polysaccharide was detected with Hotchkiss's periodic acid-Schiff's reagent method (1946), and nuclei were stained with Heidenhain's iron hematoxylin.